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  Determiantion of molecular weight
  Particle size / Mesh to microns con...
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Determiantion of molecular weight
Weight-average molecular weight (Mw), number average molecular weight (Mn) and molecular weight dispersion (Mw/Mn) of sample were measured by gel permeation chromatography (GPC). The GPC equipment consisted of TSK G3000-PWXL columns, TSP P100 pump and RI 150 refractive index detector. The eluent was 0.2 M CH3COOH/0.1 M CH3COONa. The flow rate and column’s temperature was maintained at 1.0 ml/min. The column temperature was maintained at 30°C. The sample concentration was 0.4 mg/ml. The standards used to calibrate the column were TOSOH pullulan. All data provided by the GPC system were collected and analysed using the Jiangshen Workstation software package.
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Figure 1. GPC profiles of Low Molecular Weight Chitosan

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